RNA Sequencing and Transcriptomics Intro

From RNA to insight

RNA-seq captures snapshot of expressed genes. Useful for comparing healthy vs stressed plants, or tissue types (root vs leaf).

Workflow summary

1. Extract high-quality RNA (RNase-free environment).
2. Library preparation and sequencing on NGS platform.
3. Align reads to reference genome or assemble de novo.
4. Statistical analysis for differential expression (fold-change, p-values).

Functional follow-up

Top candidate genes validated by qPCR, mutant analysis, or overexpression studies. Pathway enrichment groups genes into metabolism or stress response categories.