The invention of the compound microscope provided a valuable and durable instrument for the investigation of the inner structure of plants. Early plant morphologists, especially those studying cell structure, were handicapped as much by the lack of adequate knowledge of how to prepare specimens as they were by the imperfect microscopes of the time. A revolution in the effectiveness of microscopy occurred in the second half of the 19th century with the introduction of techniques for fixing cells and for staining their component parts. Before the development of these techniques, the cell, viewed with the microscope, appeared as a minute container with a dense portion called the nucleus. The discovery that parts of the cell respond to certain stains made observation easier. The development of techniques for preparing tissues of plants for microscopic examination was continued in the 1870s and 1880s and resulted in the gradual refinement of the field of nuclear cytology, or karyology. Chromosomes were recognized as constant structures in the life cycle of cells, and the nature and meaning of meiosis, a type of cell division in which the daughter cells have half the number of chromosomes of the parent, was discovered; without this discovery, the significance of Mendel’s laws of heredity might have gone unrecognized. Vital stains, dyes that can be used on living material, were first used in 1886 and have been greatly refined since then.
Improvement of the methodology of morphology has not been particularly rapid, even though satisfactory techniques for histology, anatomy, and cytology have been developed. The embedding of material in paraffin wax, the development of the rotary microtome for slicing very thin sections of tissue for microscope viewing, and the development of stain techniques are refinements of previously known methods. The invention of the phase microscope made possible the study of unfixed and unstained living material—hopefully nearer its natural state. The development of the electron microscope, however, has provided the plant morphologist with a new dimension of magnification of the structure of plant cells and tissues. The fine structure of the cell and of its components, such as mitochondria and the Golgi apparatus, have come under intensive study. Knowledge of the fine structure of plant cells has enabled investigators to determine the sites of important biochemical activities, especially those involved in the transfer of energy during photosynthesis and respiration. The scanning electron microscope, a relatively recent development, provides a three-dimensional image of surface structures at very great magnifications.
For experimental research on the morphogenesis of plants, isolated organs in their embryonic stage, clumps of cells, or even individual cells are grown. One of the most interesting techniques developed thus far permits the growing of plant tissue of higher plants as single cells; aeration and continuous agitation keep the cells suspended in the liquid culture medium.
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